The first-round draw for the Betfred Cup will take place on Friday evening.
The 38 SPFL teams not involved in Europe are included, as well as Cove Rangers from the Highland League and Lowland League side East Stirlingshire.
Teams will be drawn into eight groups of five teams, on a seeded basis, with fixtures announced next week.
The revamped League Cup competition, which starts on 16 July, will see the group winners and four best runners-up progress to the second round.
European representatives Celtic, Aberdeen, Hearts and Hibernian will also join at that stage.
The final will be played at Hampden Park on 27 November.
In the first-round groups, each team will play each other once, with games taking place on 16, 20, 23, 27 and 30 July, subject to live TV matches.
Any game ending in a draw will go straight to a penalties, with the winner of the penalty shoot-out awarded a bonus point.
The draw will take place at 19:30 BST on Friday.
Top seeds – North
St Johnstone, Ross County, Inverness Caledonian Thistle, Dundee
Top seeds – South
Motherwell, Partick Thistle, Hamilton Academical, Kilmarnock
Second seeds – North
Dundee United, Falkirk, Raith Rovers, Dumbarton
Second seeds – South
Rangers, Morton, St Mirren, Queen of the South
Unseeded clubs – North
Dunfermline Athletic, Alloa Athletic, Peterhead, Brechin City, East Fife, Cowdenbeath, Forfar Athletic, Elgin City, Stirling Albion, Montrose, Arbroath, Cove Rangers
Unseeded clubs – South
Ayr United, Livingston, Stranraer, Airdrieonians, Albion Rovers, Stenhousemuir, Queen’s Park, Clyde, Annan Athletic, Berwick Rangers, Edinburgh City, East Stirlingshire
